Human serum albumin

SKU: orb2563753

Description

Human Serum Albumin (HSA) is a key plasma protein that acts as a major endogenous inhibitor of amyloid-β aggregation, relevant to Alzheimer's disease research. It is widely utilized in vitro as a carrier protein in cell culture, drug delivery studies, and as a stabilizer in immunoassays and biopreservation.

Research Area

Immunology & Inflammation, Neuroscience, Protein Biochemistry, Signal Transduction

Key Properties

• CAS Number: 70024-90-7
• Target: NF-κB,Beta Amyloid,NO Synthase
• Solubility: H2O:100 mg/mL

Bioactivity

• In Vivo:
  In a study on male Swiss mouse models, Human serum albumin administered intravenously at a dose of 10 ml/kg once at 1 hour and once at 5 hours effectively mitigated the oxidative stress and nitric oxide overproduction induced by lipopolysaccharide (LPS, 50 mg/kg via intraperitoneal injection). The treatment successfully inhibited iNOS expression and formation of peroxynitrite (ONOO−), reduced the upregulation of NF-κB, and prevented the decline in vascular response to phenylephrine, muscle tone, and endothelial function caused by endotoxin.
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• In Vitro:
  Human serum albumin (HSA) at a concentration of 5 μM for 0-90 minutes can bind with Glycerol monolaurate (GML), with a dissociation constant (K d) of 1.4 μM, and it serves to protect T cell function by preventing GML from inhibiting human T cells. HSA at concentrations of 0.01, 0.05, and 0.1 μM over 0-24 hours can mitigate the GML-induced phosphorylation of AKT at threonine 308 and serine 473, as well as the formation of LAT, PLC-γ1, and AKT microclusters, while restoring the production of IFN-γ, IL-2, IL-10, and TNF-α in GML-treated cells. Western Blot analysis in activated peripheral blood T cells at a 0.05 μM concentration with incubation times of 0, 2, 5, and 15 minutes showed that it affected GML's ability to inhibit AKT phosphorylation. RT-PCR studies, using activated peripheral blood T cells with concentrations of 0.1, 0.05, and 0.005 μM over 24 hours, demonstrated the restoration of IFN-γ, IL-2, IL-10, and TNF-α production in GML-treated cells.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSA

Quick Database Links

Gene Symbol

NF-κB,Beta Amyloid,NO Synthase