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Catalog Number | orb223846 |
---|---|
Category | Proteins |
Description | Recombinant Human PTN protein |
Tested applications | SDS-PAGE |
Reactivity | Human |
Purity | > 96% by SDSPAGE and HPLC analyses. |
Conjugation | Unconjugated |
MW | 15.3 kDa |
Target | Human PTN |
Source | E. coli |
Biological Activity | Fully biologically active when compared to standard. The biological activity was measured by its ability to enhance neurite outgrowth of E16E18 rat embryonic cortical neurons, when neurons were plated on 96 well culture plates that had been precoated with 100 egl/well of a solution of 510 egg/ml |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | Sterile Filtered White lyophilized (freezedried) powder. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human PTN protein
> 96% as determined by SDSPAGE and HPLC. | |
15.3 kDa | |
E.Coli |
Unconjugated | |
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 41.4 kDa after removal of the signal peptide.The apparent molecular mass of PTN-hFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
Human | |
0.24 ng/mL-15 ng/mL | |
0.06 ng/mL |
ELISA | |
> 98% as determined by SDS-PAGE. Ni-NTA chromatography. | |
Escherichia coli |
Unconjugated | |
95% | |
17.2 kDa | |
Human Pleiotrophin Protein, His Tag, premium grade (orb1786205) is expressed from human 293 cells (HEK293). It contains AA Gly 33 - Asp 168 (Accession # P21246). |
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