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Human IL17A protein
Catalog Number: orb80077
| Catalog Number | orb80077 |
|---|---|
| Category | Proteins |
| Description | IL17A Human Recombinant produced in E.coli is homodimeric, non-glycosylated polypeptide chain containing total of 264 amino acids (2 chains of 132 aa) and having a molecular mass of 30,250 Dalton. The IL-17 is purified by proprietary chromatographic techniques. |
| Tested applications | FA, HPLC, SDS-PAGE |
| Purity | > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Conjugation | Unconjugated |
| Target | IL17 |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin 17 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Protein Sequence | MIVKAGITIP RNPGCPNSED KNFPRTVMVN LNIHNRNTNT NPKRSSDYYN RSTSPWNLHR NEDPERYPSV IWEAKCRHLG CINADGNVDY HMNSVPIQQE ILVLRREPPH CPNSFRLEKI LVSVGCTCVT PIVHHVA. |
| Biological Origin | E.coli |
| Biological Activity | The ED50 as determined by the dose-dependent induction of IL-6 in Hs68 cell line was found to be approximately 2ng/ml. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Buffer/Preservatives | Sterile filtered lyophilized (freeze-dried) powder, with 20 mM Citrate, 0.1M NaCl, pH4.5. |
| Alternative names | CTLA-8 protein, IL-17 protein, IL-17A protein, Cyt Read more... |
| Note | For research use only |
| Application notes | < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.13 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of IL-17 as Reference Standard. |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL17A protein
- Item 1 of 2
Human IL17A Protein [orb1476713]
Greater than 95% as determined by SDS-PAGE.
15.9 kDa
Baculovirus
100 μg, 20 μg, 1 mg - Item 1 of 1
Human IL17A protein [orb594787]
Greater than 95% as determined by SDS-PAGE.
15.26 kDa
E.coli
1 mg, 500 μg, 10 μg, 50 μg - Item 1 of 1
Human IL17A protein [orb594788]
Greater than 95% as determined by SDS-PAGE.
15.9kDa
Mammalian cell
1 mg, 500 μg, 10 μg, 50 μg - Item 1 of 1
Human IL17A & IL17F protein [orb594824]
Greater than 95% as determined by SDS-PAGE.
15.1kDa & 16.0 kDa
Mammalian cell
10 μg, 50 μg, 1 mg, 500 μg - Item 1 of 1
Human IL17A Protein [orb1477795]
Greater than 85% as determined by SDS-PAGE.
44.0 kDa
E.coli
20 μg, 100 μg, 1 mg
