Human IL-18BP Protein

• Catalog Number: orb257623
• Category: Proteins
• Description: Interleukin-18-binding protein (IL18BP) is also known as Tadekinig-alfa, IL18BPa, which functions as an IL18 inhibitor. IL18BP is a secreted protein which contains one Ig-like C2-type (immunoglobulin-like) domain. IL18BP does not have a transmembrane domain and hence is not anchored to the cell membrane. IL18BP is strongly expressed in heart, lung, placenta and spleen. Isoform A of IL18BP binds to IL-18 and inhibits its activity. IL18BP also functions as an inhibitor of the early TH1 cytokine response. IL18BP suppresses the production of IFN-gamma resulting in reduced T-helper type 1 immune responses.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 44.3 kDa
• Target: IL-18BP
• Source: Human IL-18BP, Fc Tag (orb257623) is expressed from human 293 cells (HEK293). It contains AA Thr 31 - Gly 194 (Accession # NP_001138529).
• Expression System: HEK293
• Biological Activity: Human IL-18BP, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free with an affinity constant of 1.71 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
• Expression Region: Thr 31 - Gly 194
• Endotoxins: 1.0 EU per μg
• NCBI: NP_001138529
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: Tris with Glycine, Arginine and NaCl, pH7.5
• Alternative names: IL18BP,IL18BPa,Tadekinig-alfa
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 44.3 kDa. The protein migrates as 56-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IL-18BP protein

Human IL-18BP, Fc Tag (Cat. No. orb257623) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free with an affinity constant of 1.71 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).