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Catalog Number | orb334903 |
---|---|
Category | Proteins |
Description | Interleukin-10 (IL-10) is also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. Mature human IL-10 shares 72% - 86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is capable of inhibiting synthesis of pro-inflammatory cytokines such as IFN-γ, IL-2, IL-3, TNFα and GM-CSF made by cells such as macrophages and regulatory T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production. Knockout studies suggested the function of Interleukin-10 / IL-10 as an essential immunoregulator in the intestinal tract. Patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant interleukin-10, showing the importance of interleukin-10 for counteracting excessive immunity in the human body. |
Reactivity | Human |
Tag | N-6×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 19.5 kDa |
Target | IL-10 |
Source | Human IL-10, His Tag (orb334903) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # NP_000563.1). |
Expression System | HEK293 |
Biological Activity | Immobilized Human IL-10, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-10 R alpha, Fc, with a linear range of 0.2-10 ng/mL (QC tested). |
Expression Region | Ser 19 - Asn 178 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000563.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | CSIF,IL-10,IL10A,TGIF,B-TCGF,GVHDS,MGC126450,MGC12 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 19.5 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL-10 protein
Immobilized Human IL-10, His Tag (Cat. No. orb334903) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-10 R alpha, Fc, Avitag with a linear range of 0.2-10 ng/mL (QC tested).
> 95% as determined by SDS-PAGE and HPLC. | |
18.6 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
11.2 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
16.9 kDa | |
E.coli |
Unconjugated | |
95% | |
21.1 kDa | |
Human IL-29, His Tag (orb257605) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - Thr 200 (Accession # NP_742152.1). |
Unconjugated | |
90% | |
27.1 kDa | |
Human IL-20 R alpha Protein, His Tag (orb1184776) is expressed from human 293 cells (HEK293). It contains AA Val 30 - Lys 250 (Accession # Q9UHF4-1). |
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