Human IGFBP-4 Protein

• Catalog Number: orb348805
• Category: Proteins
• Description: Insulin-like growth factor-binding protein 4 (IGFBP-4) is also known as IGF-binding protein 4, which contains an IGFBP domain and a thyroglobulin type-I domain. IGFBP-4 binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. IGFBP-4 is secreted by all colon cancer cells.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 27.9 kDa
• Target: IGFBP-4
• Source: Human IGFBP-4, His Tag (orb348805) is expressed from human 293 cells (HEK293). It contains AA Asp 22 - Glu 258 (Accession # P22692-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human IGFBP-4, His Tag at 2 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag with a linear range of 1-16 ng/mL (QC tested).
• Expression Region: Asp 22 - Glu 258
• Endotoxins: 1.0 EU per μg
• NCBI: NP_001543.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: IGFBP4,IBP-4
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.9 kDa. The protein migrates as 33-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IGFBP-4 protein

Immobilized Human IGFBP-4, His Tag (Cat. No. orb348805) at 2 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag (Cat. No. orb257564) with a linear range of 1-16 ng/mL (QC tested).