Human IGF-II Protein

• Catalog Number: orb257566
• Category: Proteins
• Description: Insulin-like growth factor 2 (IGF-2) is also known as Somatomedin-A, IGF-II, PP9974, and is one of three protein hormones that share structural similarity to insulin. IGF-2 exerts its effects by binding to the IGF-1 receptor. IGF2 may also bind to the IGF-2 receptor (also called the cation-independent mannose 6-phosphate receptor), which acts as a signalling antagonist; that is, to prevent IGF2 responses. The major role of IGF2 is as a growth promoting hormone during gestation. In the process of Folliculogenesis, IGF2 is created by Theca cells to act in an autocrine manner on the theca cells themselves, and in a paracrine manner on Granulosa cells in the ovary. IGF2 promotes granulosa cell proliferation during the follicular phase of the menstrual cycle, acting alongside Follicle Stimulating Hormone (FSH). After ovulation has occurred, IGF-2 promotes progesterone secretion during the luteal phase of the menstrual cycle together with Luteinizing Hormone (LH). Thus, IGF2 acts as a Co-hormone together with both FSH and LH. IGF-2 may play a key role in memory and could potentially be used to treat Alzheimer's Disease. It is sometimes produced in excess in islet cell tumours, causing hypoglycemia. Doege-Potter syndrome is a paraneoplastic syndrome in which hypoglycemia is associated with the presence of one or more non-islet fibrous tumors in the pleural cavity. has been shown to interact with IGFBP3 and Transferrin.
• Reactivity: Human
• Tag: N-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 34.4 kDa
• Target: IGF-II
• Source: Human IGF-II, Fc Tag (orb257566) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Glu 91 (Accession # P01344-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human IGFBP-3, His Tag at 0.5 μg/mL (100 μL/well) can bind Human IGF-II, Fc Tag with a linear range of 1.2-39 ng/mL (QC tested).
• Expression Region: Ala 25 - Glu 91
• Endotoxins: 1.0 EU per μg
• NCBI: P01344
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, pH7.5
• Alternative names: IGF2,C11orf43,FLJ22066,FLJ44734,IGF-II,PP9974
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 34.4 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human IGF-II protein

Immobilized Human IGFBP-3, His Tag (Cat. No. orb1146966) at 0.5 μg/mL (100 μL/well) can bind Human IGF-II, Fc Tag (Cat. No. orb257566) with a linear range of 1.2-39 ng/mL (QC tested).