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Human FGF10 protein
Catalog Number: orb82610
| Catalog Number | orb82610 |
|---|---|
| Category | Proteins |
| Description | FGF10 (also known as KGF2) was originally identified from rat embryos by homology-based polymerase chain reaction. Human and mouse KGF-2 were subsequently cloned. The human FGF10 cDNA encodes a 208 amino acid residue protein with a hydrophobic amino-terminal signal peptide. Human FGF10 shares approximately 92% and 95% amino acid sequence identity with mouse and rat FGF10, respectively. Among the FGF family members,FGF10 is most closely related to FGF-7. The expression of FGF10 transcripts has been shown to be most abundant in the embryo and adult lung. Recombinant FGF10 preparations have been shown to be mitogenic for epithelial and epidermal cells but not fibroblasts. Based on its in vitro biological activities and in vivo expression pattern, FGF10 has been proposed to play unique roles in the brain, in lung development, wound healing and limb bud formation. |
| Target | FGF10 |
| Conjugation | Unconjugated |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered concentrated (1mg/ml) solution in PBS, pH 7.4. We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. |
| Purity | > 96% pure by SDS-PAGE and HPLC analyses. |
| Protein Sequence | MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS |
| MW | 19.3 kDa |
| Tested applications | HPLC, SDS-PAGE |
| Application notes | Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions. |
| Biological Origin | E.coli |
| Biological Activity | The biological activity was determined by the does-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors yielding an ED50 < 0.5ng/ml., corresponding to a specific activity of 2.0 x 106 Units/mg. Less than 1EU/μg of rHuKGF-2 as determined by LAL method. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Alternative names | Keratinocye Growth Factor-2 |
| Note | For research use only |
SDS-PAGE analysis of Human FGF10 protein
- Item 1 of 1
Human FGF10 protein [orb244206]
Greater than 90% as determined by SDS-PAGE.
46.3 kDa
E.coli
20 μg, 100 μg, 1 mg - Item 1 of 1
Human FGF10 protein (Active) [orb359155]
> 97% as determined by SDS-PAGE and HPLC.
19.1 kDa
E.Coli
5 μg, 100 μg, 500 μg - Item 1 of 1
Recombinant human FGF10 protein [orb1516459]
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Due to glycosylation, the protein migrates to 25 kDa based on Tris-Bis PAGE result.
100 μg 
Human FGF10 protein [orb706937]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
E. coli
500 μg, 50 μg, 10 μgRecombinant Human FGF10 Protein, N-His [orb2833228]
> 90% as determined by SDS-PAGE.
19.06 kDa
100 μg, 50 μg, 20 μg
