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Catalog Number | orb425458 |
---|---|
Category | Proteins |
Description | Recombinant of human FEN1 protein |
Form/Appearance | Sterile filtered colorless solution. |
Buffer/Preservatives | The protein contains 20mM Tris-HCl buffer pH-8.0, 1mM DTT, 10% glycerol. |
Purity | Greater than 90.0% as determined by SDS-PAGE. |
Protein Sequence | MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK |
Application notes | Enzymes |
Source | Escherichia Coli |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles |
Alternative names | FEN-1, MF1, RAD2, Maturation Factor-1, MF-1, Flap Read more... |
Note | For research use only |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
> 90% as determined by SDS-PAGE. | |
40.02 kDa |
ELISA, WB | |
Greater than 95% by SDS-PAGE gel analyses | |
56.3 kDa | |
E.Coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human FEN1(Met1-Lys380) was fused with the C-terminal His Tag and expressed in E. coli. |