Human Collagen VIII protein

SKU: orb391503

Description

Recombinant human Collagen VIII protein is produced by our Mammalian expression system and the target gene encoding Gly28-Met744 is expressed with a 6His tag at the C-terminus.

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Reactivity: Human
• Protein Sequence: GAYYGIKPLP PQIPPQMPPQ IPQYQPLGQQ VPHMPLAKDG LAMGKEMPHL QYGKEYPHLP QYMKEIQPAP RMGKEAVPKK GKEIPLASLR GEQGPRGEPG PRGPPGPPGL PGHGIPGIKG KPGPQGYPGV GKPGMPGMPG KPGAMGMPGA KGEIGQKGEI GPMGIPGPQG PPGPHGLPGI GKPGGPGLPG QPGPKGDRGP KGLPGPQGLR GPKGDKGFGM PGAPGVKGPP GMHGPPGPVG LPGVGKPGVT GFPGPQGPLG KPGAPGEPGP QGPIGVPGVQ GPPGIPGIGK PGQDGIPGQP GFPGGKGEQG LPGLPGPPGL PGIGKPGFPG PKGDRGMGGV PGALGPRGEK GPIGAPGIGG PPGEPGLPGI PGPMGPPGAI GFPGPKGEGG IVGPQGPPGP KGEPGLQGFP GKPGFLGEVG PPGMRGLPGP IGPKGEAGQK GVPGLPGVPG LLGPKGEPGI PGDQGLQGPP GIPGIGGPSG PIGPPGIPGP KGEPGLPGPP GFPGIGKPGV AGLHGPPGKP GALGPQGQPG LPGPPGPPGP PGPPAVMPPT PPPQGEYLPD MGLGIDGVKP PHAYGAKKGK NGGPAYEMPA FTAELTAPFP PVGAPVKFNK LLYNGRQNYN PQTGIFTCEV PGVYYFAYHV HCKGGNVWVA LFKNNEPVMY TYDEYKKGFL DQASGSAVLL LRPGDRVFLQ MPSEQAAGLY AGQYVHSSFS GYLLYPMVDH HHHHH
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
• Disclaimer: For research use only

Alternative Names

C3orf7; Collagen alpha-1(VIII) chain; Endothelial collagen

Quick Database Links

UniProt

P27658

NCBI Gene ID

1295