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Catalog Number | orb391503 |
---|---|
Category | Proteins |
Description | Recombinant human Collagen VIII protein is produced by our Mammalian expression system and the target gene encoding Gly28-Met744 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | COL8A1 |
Entrez | 1295 |
UniProt ID | P27658 |
Protein Sequence | GAYYGIKPLP PQIPPQMPPQ IPQYQPLGQQ VPHMPLAKDG LAMGKEMPHL QYGKEYPHLP QYMKEIQPAP RMGKEAVPKK GKEIPLASLR GEQGPRGEPG PRGPPGPPGL PGHGIPGIKG KPGPQGYPGV GKPGMPGMPG KPGAMGMPGA KGEIGQKGEI GPMGIPGPQG PPGPHGLPGI GKPGGPGLPG QPGPKGDRGP KGLPGPQGLR GPKGDKGFGM PGAPGVKGPP GMHGPPGPVG LPGVGKPGVT GFPGPQGPLG KPGAPGEPGP QGPIGVPGVQ GPPGIPGIGK PGQDGIPGQP GFPGGKGEQG LPGLPGPPGL PGIGKPGFPG PKGDRGMGGV PGALGPRGEK GPIGAPGIGG PPGEPGLPGI PGPMGPPGAI GFPGPKGEGG IVGPQGPPGP KGEPGLQGFP GKPGFLGEVG PPGMRGLPGP IGPKGEAGQK GVPGLPGVPG LLGPKGEPGI PGDQGLQGPP GIPGIGGPSG PIGPPGIPGP KGEPGLPGPP GFPGIGKPGV AGLHGPPGKP GALGPQGQPG LPGPPGPPGP PGPPAVMPPT PPPQGEYLPD MGLGIDGVKP PHAYGAKKGK NGGPAYEMPA FTAELTAPFP PVGAPVKFNK LLYNGRQNYN PQTGIFTCEV PGVYYFAYHV HCKGGNVWVA LFKNNEPVMY TYDEYKKGFL DQASGSAVLL LRPGDRVFLQ MPSEQAAGLY AGQYVHSSFS GYLLYPMVDH HHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, pH 8.0. |
Alternative names | C3orf7; Collagen alpha-1(VIII) chain; Endothelial Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
31.9 kDa | |
E.coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human COL8A1(Gly28-Met744) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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