Human Collagen IV Protein

SKU: orb81035

Description

Recombinant of Human Collagen IV protein

Images & Validation

• Application Notes:
  Coating concentration: 0.12-0.5 μg/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups. Note: It has been reported that the immunodominant epitope of GBM is a cryptic epitope that is not easily accessible to the corresponding autoantibodies. It is necessary to treat the protein under nonreducing conditions with a denaturant such as urea to unmask the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B. and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298)

Key Properties

• Source: Sf9 insect cells
• Purity: Greater than 95.0% as determined by SDS-PAGE.

Storage & Handling

• Storage: Stability: Recommendations for storage buffer: neutral to slightly alkaline pH and 4M urea as dissociating agent. Storage temperature: -70°C to -80°C. Please prevent freeze-thaw cycles
• Form/Appearance: Sterile Filtered clear solution.
• Buffer/Preservatives: The protein solution contains 20mM Hepes, pH 8.0 and 4M Urea.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Collagen alpha-3(IV) chain, Goodpasture antigen, COL4A3, Glomerular Basal Membrane, GBM.