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Human CGRP protein

SKU: orb391345

Description

Recombinant human CGRP protein is produced by our E. coli expression system and the target gene encoding Met1-Ala127 is expressed with a GST tag at the N-terminus.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
ReactivityHuman
Protein SequenceMSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSENLY FQGHMGFRKF SPFLALSILV LYQAGSLQAA PFRSALESSP DPATLSKEDA RLLLAALVQD YVQMKASELK QEQETQGSSS AAQKRACNTA TCVTHRLAGL LSRSGGMVKS NFVPTNVGSK AFGRRRRDLQ A
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceSupplied as a 0.2 μM filtered solution of PBS, 50% glycerol, pH 7.4.
DisclaimerFor research use only

Alternative Names

CALC2; Calcitonin gene-related peptide 2; Beta-type CGRP; Beta-CGRP; Calcitonin gene-related peptide II; CGRP-II

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human CGRP protein (orb391345)

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Available Sizes

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10 μg
$ 140.00
50 μg
$ 320.00
500 μg
$ 1,870.00
DispatchUsually dispatched within 5-10 working days
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