Human S100 Calcium Binding Protein A12 (S100A12) ELISA Kit

SKU: orb774938

Description

The Human S100 Calcium Binding Protein A12 (S100A12) ELISA Kit uses a sandwich enzyme immunoassay to quantify S100 Calcium Binding Protein A12 (S100A12) in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 78.13-5000 pg/mL and a sensitivity of 31 pg/mL.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S100A12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S100A12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human S100A12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human S100A12 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 78.13-5000 pg/mL
• Sensitivity: 31 pg/mL
• Concentration: 5000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

CGRP ELISA Kit, P6 ELISA Kit, CAAF1 ELISA Kit, CAGC ELISA Kit, ENRAGE ELISA Kit, MRP6 ELISA Kit, Calgranulin C ELISA Kit, Calcium-binding protein in amniotic fluid 1 ELISA Kit, Extracellular newly identified RAGE-binding ELISA Kit, Migration inhibitory factor-related 6 ELISA Kit

Quick Database Links

UniProt

P80511