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    HSPA8 antibody

    Catalog Number: orb214062

    DispatchUsually dispatched within 5-10 working days
    $ 337.00
    Catalog Numberorb214062
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to HSPA8
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, WB
    ReactivityHuman, Mouse, Rat
    ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSC70. The exact sequence is proprietary.
    Dilution rangeWB: 1-500-1-1000, IP: 1-10-1-100
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetHSPA8
    Entrez3312, 15481, 24468
    UniProt IDP63018, P11142, P63017
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesanti HSC70 antibody, anti HSP73 antibody, anti HSP
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    HSPA8 antibody

    Western blot analysis of HSC70 expression in HEK293T (A), Hela (B), H460 (C), mouse heart (D), mouse brain (E), rat heart (F), rat muscle (G) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 70 kD)

    HSPA8 antibody

    Immunofluorescent analysis of HSC70 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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      Bovine, Goat, Human, Mouse, Porcine, Rat, Sheep, Zebrafish

      Goat, Human, Mouse, Porcine, Rat, Sheep, Zebrafish

      Rabbit

      Polyclonal

      Unconjugated

      100 μl
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