Hsc70/HSPA8 Antibody

• Catalog Number: orb315149
• Category: Antibodies
• Description: Hsc70/HSPA8 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Hsc70 recombinant protein (Position: Q520-A614). Human Hsc70 shares 98.9% amino acid (aa) sequence identity with both mouse and rat Hsc70.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatImmunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 70898 MW
• UniProt ID: P11142
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Heat shock cognate 71 kDa protein;Heat shock 70 kD
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of K562 cells using anti-Hsc70 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Hsc70 using anti-Hsc70 antibody.Lane 1:human HeLa cell; 2:human HepG2 cell; 3:human CACO-2 cell;4:human A549 cell;5:human THP-1 cell;6:human K562 cell;7:human PC-3 cell;8:human U87MG cell.

WB analysis of Hsc70 using anti-Hsc70 antibody.Lane 1:rat liver tissue; 2:rat spleen tissue; 3:rat brain tissue;4:rat kidney tissue;5:mouse liver tissue;6:mouse spleen tissue;7:mouse brain tissue;8:mouse RAW264.7 cell.

IF analysis of Hsc70 using anti-Hsc70 antibody. Hsc70 was detected in immunocytochemical section of U20S cells.

IHC analysis of Hsc70 using anti-Hsc70 antibody. Hsc70 was detected in a paraffin-embedded section of mouse kidney tissue.

IHC analysis of Hsc70 using anti-Hsc70 antibody. Hsc70 was detected in a paraffin-embedded section of human mammary cancer tissue.

IHC analysis of Hsc70 using anti-Hsc70 antibody. Hsc70 was detected in a paraffin-embedded section of rat kidney tissue.