HSP90β rabbit pAb

• Catalog Number: orb765451
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HSP90 beta
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human HSP90B. AA range:226-275
• MW: 90
• Tested applications: ELISA, IF, IHC-P, IP, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-HSP90AB1 antibody, anti-HSP90B antibody, anti
• Note: For research use only

Western Blot analysis of various cells using HSP90β Polyclonal Antibody diluted at 1:2000.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western blot analysis of HSP90B Antibody. The lane on the right is blocked with the HSP90B peptide.

Immunofluorescence analysis of HeLa cells, using HSP90B Antibody. The picture on the right is blocked with the synthesized peptide.