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| Catalog Number | orb764382 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to HSP90beta (phospho-Ser254) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Monkey, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSP90B around the phosphorylation site of Ser254. AA range:226-275 |
| MW | 90 |
| Tested applications | ELISA, IF, IHC-P, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-HSP90AB1 antibody, anti-HSP90B antibody, anti Read more... |
| Note | For research use only |
Western Blot analysis of various cells using Phospho-HSP90β (S254) Polyclonal Antibody.
Western Blot analysis of HepG2 cells using Phospho-HSP90β (S254) Polyclonal Antibody.
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HSP90B (Phospho-Ser254) Antibody.
