HSD3B1+ HSD3B2 Rabbit Polyclonal Antibody

SKU: orb526567

Description

HSD3B1+ HSD3B2 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Equine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HSD3B1+ HSD3B2 (101-200/372aa)
• Target: HSD3B1
• Molecular Weight: 41 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/1ml
• Disclaimer: For research use only

Alternative Names

3 beta and steroid delta isomerase 1; 3 beta and steroid hydroxy delta 5 steroid dehydrogenase; 3 beta HSD I; 3BH; 3BHSD; HGNC:5217; HSD3B; HSD3B1; HSDB3; HSDB3A; Hydroxy delta 5 steroid dehydrogenase 3 beta and steroid delta isomerase 1; Hydroxy delta 5 steroid dehydrogenase; trophoblast antigen; 3BHS1_HUMAN; 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase type 1; 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase type I; Short=3-beta-HSD I; Trophoblast antigen FDO161G; 3-beta-hydroxy-delta(5)-steroid dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid delta-isomerase; delta-5-3-ketosteroid isomerase.

Paraformaldehyde-fixed, paraffin embedded (human adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (HSD3B1 + HSD3B2) Polyclonal Antibody, Unconjugated (orb526567) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (HSD3B1 + HSD3B2) Polyclonal Antibody, Unconjugated (orb526567) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (HSD3B1 + HSD3B2) Polyclonal Antibody, Unconjugated (orb526567) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (HSD3B1 + HSD3B2) Polyclonal Antibody, Unconjugated (orb526567) at 1:300 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Adrenal gland tissue lysates, Lane 2: Rat Adrenal gland tissue lysates, Primary: Anti-HSD3B1 + HSD3B2 (orb526567) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 41 kDa, Observed band size: 41 kDa.

Quick Database Links

Gene Symbol

HSD3B1

UniProt

P14060