HPV-16 L1 Antibody

• Catalog Number: orb2641941
• Category: Antibodies
• Description: Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MCAPL1
• Tested applications: IHC-P
• Reactivity: Virus
• Isotype: Mouse IgG2a, kappa
• Immunogen: Human papilloma virus type 16, major capsid protein L1, was used as the immunogen for the HPV-16 L1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This HPV-16 L1 antibody is available for research use only.
• UniProt ID: P03101
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the HPV-16 L1 antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human cervix with HPV-16 L1 antibody (clone MCAPL1). FFPE testing requires sections to be boiled in pH9 10 mM Tris with 1 mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.