HLA-G antibody

• Catalog Number: orb44439
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-G.
• Clonality: Monoclonal
• Clone Number: MEM-G/9
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Recombinant human HLA-G refolded with beta2-microglobulin and peptide.
• Concentration: 0.1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-5 μg/ml; positive control: JEG-3 human choriocarcinoma cell line.
• Purity: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC
• Target: HLA-G
• Entrez: 3135
• UniProt ID: P17693
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-G antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml; positive control: JEG-3 human choriocarcinoma cell line.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC (cat. no. orb44429).

Separation of LCL 721.221 HLA-G transfected cells stained using anti-human HLA-G (MEM-G/9) Alexa Fluor® 647 antibody antibody (10 µl reagent per million cells in 100 µl of cell suspension, red-filled) from LCL 721.221 HLA-G transfected cells stained using mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 647 antibody (concentration in sample 1 µg/ml, same as CD135 APC concentration, black-dashed) in flow cytometry analysis (surface staining) of LCL 721.221 HLA-G transfected cells suspension.