HLA-G antibody

• Catalog Number: orb44426
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-G.
• Clonality: Monoclonal
• Clone Number: MEM-G/1
• Tested applications: IHC-Fr, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Denatured bacterially expressed recombinant human HLA-G heavy chain.
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Recommended dilution: 1:60 to 1:100, incubation: 1 h at RT; positive tissue: human placenta - extravillous cytotrophoblast, heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven).Western blotting: Recommended dilution 1-2 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HLA-G
• Entrez: 3135
• UniProt ID: P17693
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-G antibody
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 1:60 to 1:100, incubation: 1 h at RT; positive tissue: human placenta - extravillous cytotrophoblast, heat retrieval in 0.01M citrate buffer (4x2 min. in microwave oven).
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry staining with anti-human HLA-G (MEM-G/1) - pulmonary disseases (paraffin-embedded sections). The antibody MEM-G/1 stains infiltrating macrophages in pulmonary diseases. In the top left corner see the detail of macrophage.

Immunohistochemistry staining with anti-human HLA-G (MEM-G/1) of first trimester placenta (paraffin-embedded sections).

Western blotting analysis of human HLA-G using mouse monoclonal antibody MEM-G/1 on lysates of JEG-3 cell line and LNCaP cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-HLA-G followed by IRDye800-conjugated anti-mouse secondary antibody. HLA-G was detected at approximately 40 kDa.