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Catalog Number | orb44431 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to HLA-G. |
Clonality | Monoclonal |
Clone Number | MEM-G/2 |
Tested applications | IHC-Fr, IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | Bacterially expressed recombinant HLA-G heavy chain (denatured). |
Concentration | 1 mg/ml |
Dilution range | Western blotting: Recommended dilution 1-2 μg/ml. |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | HLA-G |
Entrez | 3135 |
UniProt ID | P17693 |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-HLA-G antibody Read more... |
Note | For research use only |
Application notes | Western blotting: Recommended dilution 1-2 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blotting analysis of HLA-G in LCL-HLA-G transfectants using anti-HLA-G (MEM-G/2).
Immunohistochemistry staining of kidney allograft biopsy (paraffin-embedded sections) with anti-human HLA-G (MEM-G/2).
Western blotting analysis of human HLA-G using mouse monoclonal antibody MEM-G/2 on lysates of JEG-3 cell line and LNCaP cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-HLA-G followed by IRDye800-conjugated anti-mouse secondary antibody. HLA-G was detected at approximately 40 kDa.
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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