HLA-DRB1 Antibody (MHC II)

• Catalog Number: orb2638585
• Category: Antibodies
• Description: This mAb reacts with the HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. It binds a conformational epitope on HLA-DR, which depends on the correct folding of the alpha/beta heterodimer. This mAb has been reported to block mixed lymphocyte reactions. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kD alpha (heavy) chain and a 28kD beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: L243
• Tested applications: FACS, IF
• Reactivity: Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DRB1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This HLA-DRB1 antibody is available for research use only.
• UniProt ID: P01911
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the HLA-DRB1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE Analysis of Purified, BSA-Free HLA-DRB1 Antibody (clone L243). Confirmation of Integrity and Purity of the Antibody.