HLA-DRB1 Antibody (MHC II)

• Catalog Number: orb317069
• Category: Antibodies
• Description: HLA-DRB1 binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: LN3
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DRB1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-3ug/ml,Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This HLA-DRB1 antibody is available for research use only.
• UniProt ID: P01911
• Storage: Store the HLA-DRB1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the HLA-DRB1 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of human Ramos cells with HLA-DRB1 antibody (clone LN3, green) and Reddot nuclear stain (red).

IHC staining of FFPE human tonsil tissue with HLA-DRB1 antibody (clone LN3). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human histiocytoma tissue with HLA-DRB1 antibody (clone LN3). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Ramos cell lysate with HLA-DRB1 antibody (clone LN3). Expected molecular weight ~30 kDa.

Western blot testing of human Ramos and spleen cell lysate with HLA-DRB1 antibody (clone LN3). Expected molecular weight ~30 kDa.

Flow cytometry staining of human Raji cells with HLA-DRB1 antibody; Red = isotype control, Blue = HLA-DRB1 antibody.