HLA-DR Antibody (MHC II)

SKU: orb317069

Description

HLA-DR binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. [UniProt]

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-3ug/ml,Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the HLA-DR antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: LN3
• Immunogen: Activated human peripheral blood mononuclear cells were used as the immunogen for the HLA-DR antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Immunofluorescent staining of human Ramos cells with HLA-DRB1 antibody (clone LN3, green) and Reddot nuclear stain (red).

IHC staining of FFPE human tonsil tissue with HLA-DRB1 antibody (clone LN3). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human histiocytoma tissue with HLA-DRB1 antibody (clone LN3). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human Ramos cell lysate with HLA-DRB1 antibody (clone LN3). Expected molecular weight ~30 kDa.

Western blot testing of human Ramos and spleen cell lysate with HLA-DRB1 antibody (clone LN3). Expected molecular weight ~30 kDa.

Flow cytometry staining of human Raji cells with HLA-DRB1 antibody; Red = isotype control, Blue = HLA-DRB1 antibody.

Quick Database Links

UniProt

P01911