HLA-DR antibody

• Catalog Number: orb154429
• Category: Antibodies
• Description: Mouse monoclonal antibody to HLA-DR which belongs to the MHC class II family. It localized in endoplasmic reticulum membrane, golgi apparatus. It binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC). HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4+ T lymphocytes.
• Clonality: Monoclonal
• Clone Number: L243
• Tested applications: FA, FC, ICC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Canine, Human, Primate
• Isotype: Mouse IgG2a kappa
• Immunogen: Human B lymphocytes
• Concentration: 1 mg/ml
• Dilution range: Functional application: Blocking. Flow cytometry: Recommended dilution: 1-4 µg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: HLA-DR
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Separation of human HLA-DR positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-DR (L243) purified antibody (concentration in sample 0.3 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR (L243) purified antibody (concentration in sample 0.3 μg/ml) GAM APC.