HLA-DR antibody (FITC)

• Catalog Number: orb44043
• Category: Antibodies
• Description: Mouse Monoclonal to HLA-DR.
• Clonality: Monoclonal
• Clone Number: MEM-12
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: thymocyte membrane
• Dilution range: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Purity: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC
• Target: HLA-DR
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-HLA-DR antibody
• Note: For research use only
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human HLA-DR (MEM-12) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Separation of human HLA-DR positive CD3 negative lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA-DR (MEM-12) APC antibody (20 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA-DR (MEM-12) FITC antibody (20 µl reagent / 100 µl of peripheral whole blood).