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Catalog Number | orb198114 |
---|---|
Category | Antibodies |
Description | Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 423L |
Tested applications | FACS |
Reactivity | Human |
Isotype | IgG2a |
Concentration | Titered for flow cytometry |
Conjugation | FITC |
Hazard Information | This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. |
UniProt ID | P04233 |
Source | Derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells of BALB/c mice immunized with human lymphoblastoid B-cell line RPMI 8866 |
Storage | Product should be stored at 4-8°C. DO NOT FREEZE |
Alternative names | HLA-DR* FITC Read more... |
Note | For research use only |
Application notes | Flow Cytometry PBMC: Add 10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of Peripheral blood lymphocytes using HLA-DR antibody (FITC)
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