HLA-B antibody

• Catalog Number: orb318746
• Category: Antibodies
• Description: Goat polyclonal antibody to HLA-B
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 40.5
• Target: HLA-B (aa132-143)
• Entrez: 3106
• Protein Sequence: RLLRGHDQYAYD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti HLA-B antibody, anti major histocompatibility
• Note: For research use only
• Application notes: WB: Approx 40+45kDa bands observed in lysates of cell line Jurkat (calculated MW of 40.5kDa according to NP_005505.2). The observed 45kDa band corresponds to the glycosylated form.Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 2.5µg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Jurkat lysate using HLA-B antibody

Immunohistochemical staining of Human Tonsil using HLA-B antibody