BAT3/BAG6 Antibody

• Catalog Number: orb527016
• Category: Antibodies
• Description: BAT3/BAG6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human BAT3/BAG6 recombinant protein (D15-A88).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 150 kDa
• UniProt ID: P46379
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Large proline-rich protein BAG6; BAG family molecu
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SH-SY5Y cells using anti-BAT3/BAG6 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of BAT3/BAG6 using anti-BAT3/BAG6 antibody.Lane 1:human HeLa cell;2:human U87 cell;3:human SH-SY5Y cell;4:rat brain tissue;5:rat C6 cell;6:mouse brain tissue;7:mouse Neuro-2a cell.

IF analysis of BAT3/BAG6 using anti-BAT3/BAG6 antibody and anti-Beta Tubulin antibody. BAT3/BAG6 was detected in immunocytochemical section of PC3 cell.

IHC analysis of BAT3/BAG6 using anti-BAT3/BAG6 antibody. BAT3/BAG6 was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of BAT3/BAG6 using anti-BAT3/BAG6 antibody. BAT3/BAG6 was detected in a paraffin-embedded section of human ovarian serous adenocarcinoma tissue.