Histone H2A.X (Phospho-S139) antibody

• Catalog Number: orb334667
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Histone H2A.X (Phospho-S139)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S139 of human Histone H2A.X protein. The exact sequence is proprietary.
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: H2AFX
• Entrez: 15270, 3014
• UniProt ID: P16104, P27661
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti H2AX antibody, anti Histone H2AX antibody, an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H2A.X (Phospho-S139) expression in Hela (A) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 15 kD)

Immunohistochemical analysis of Histone H2A.X (Phospho-S139) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.