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Catalog Number | orb416660 |
---|---|
Category | Antibodies |
Description | H2AFX (phospho-S139) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ChIP, ELISA, IF, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Peptide sequence around site of Phospho-Ser (139) derived from Human Histone H2AX |
Dilution range | WB:1:500-5000, IF:1:50-200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | H2AFX |
UniProt ID | P16104 |
Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Buffer/Preservatives | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol |
Alternative names | Anti-Histone H2AX antibody, anti- H2a/x antibody, Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- H2AFX (phospho-S139) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-t
Western blot aanalysis of Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysateusing H2AFX (phospho-S139) antibody
Immunofluorescence staining of Hela using H2AFX (phospho-S139) antibody
FC, IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
IF, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
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