Histone H2A.X (phospho-S139) antibody

• Catalog Number: orb1474191
• Category: Antibodies
• Description: Mouse monoclonal antibody to Histone H2A.X (phospho-S139)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S139 of human Histone H2A.X protein. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IF/IC (1/50 - 1/100)
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Conjugation: Unconjugated
• Target: H2AX
• Entrez: 15270, 3014
• UniProt ID: P16104, P27661
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: H2AX; Histone H2AX; H2a/x; Histone H2A.X
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Histone H2A.X (Phospho-S139) expression in NIH3T3 treated with Hydroxyurea (A) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 15 kD)

Immunofluorescent analysis of Histone H2A.X (Phospho-S139) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.