Histone H1 Antibody

• Catalog Number: orb749550
• Category: Antibodies
• Description: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody.
• UniProt ID: P07305
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Application notes: Optimal dilution of the Histone antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: AE-4
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Histone antibody is available for research use only.
• Note: For research use only

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Histone H1 antibody (AE-4)

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with Histone antibody (AE-4)

IHC: FFPE human angiosarcoma tested with Histone antibody (AE-4)

IHC: FFPE human testicular carcinoma tested with Histone antibody (AE-4)

IHC: FFPE human pancreas tested with Histone antibody (AE-4)

Western blot testing of human heart tissue lysate with Histone antibody (clone AE-4). Observed molecular weight ~22/30-33kDa (unmodified/modified).

Flow cytometry testing of permeabilized human HeLa cells with Histone antibody (clone AE-4); Red = isotype control, Blue = Histone antibody.

Immunofluorescent staining of PFA-fixed human HeLa cells with Histone antibody (green, clone AE-4) and phalloidin (red).