HIF1 alpha antibody

• Catalog Number: orb334997
• Category: Antibodies
• Description: Goat polyclonal to alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in apoptosis, angiogenesis, energy metabolism, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in tumour angiogenesis, embryonic vascularization, and pathophysiology of ischemic disease.
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep
• Isotype: IgG
• Immunogen: Purified recombinant peptide derived from within residues 470 to 590 aa of human HIF1a produced in E. coli.
• Concentration: 3 mg/ml
• Dilution range: WB:1:500-1:2,000
• Form/Appearance: Polyclonal antibody supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
• Conjugation: Unconjugated
• Target: HIF1a
• Storage: Store at -20°C for long-term storage. Store at 2-8°C for up to one month.
• Buffer/Preservatives: PBS, 20% glycerol and 0.05% sodium azide
• Alternative names: alpha subunit (basic helix-loop-helix transcriptio
• Note: For research use only
• Application notes: The antibody solution should be gently mixed before use.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of staining of HT1080 cells in the presence of CoCl2 lysate using HIF1 alpha antibody