HIF1 alpha antibody

• Catalog Number: orb334653
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HIF1 alpha
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Mouse, Rat
• Immunogen: Recombinant protein corresponding to mouse HIF1 alpha.
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:200-1:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: HIF1A
• Entrez: 15251, 29560
• UniProt ID: Q61221, O35800
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-ARNT interacting protein antibody, anti-HIF 1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HIF1 alpha expression in mouse liver (A), rat liver (B) whole cell lysates. (Predicted band size: 93 kD; Observed band size: 92-130 kD)

Immunohistochemical analysis of HIF1 alpha staining in mouse kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.