HGF Antibody

• Catalog Number: orb1266987
• Category: Antibodies
• Description: HGF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This HGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-554 amino acids from the C-terminal region of human HGF.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 83 kDa
• Target: HGF
• UniProt ID: P14210
• NCBI: P14210
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Hepatocyte growth factor, Hepatopoietin-A, Scatter
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Antibody is used in Western blot to detect HGF in Ramos tissue lysate. HGF (arrow) was detected using the purified Pab.

Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HGF antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.