You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1806454 |
---|---|
Category | Antibodies |
Description | Met Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Tested applications | FC, ICC, IHC, IP, WB |
Reactivity | Human |
Immunogen | between 1340 and 1390 (C-term) |
Concentration | 1000 µg/ml |
Dilution range | WB: 1:1000, IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Met |
UniProt ID | P08581 |
Storage | Shelf life: 1 year from date of receipt. Storage: 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free |
Alternative names | HGF/SF receptor; tyrosine-protein kinase Met; SF r Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human MET by western blot. Samples: Whole cell lysate (10 µg) from GaMG, T-47D, HT-29, OVCAR-8, and 786-O cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human MET by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human MET by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human MET (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human MET by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from GaMG cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MET recombinant monoclonal antibody (orb1806454) used for IP at 6 µl/mg lysate.
Detection of human MET by immunocytochemistry. Sample: FFPE section of GaMG cells. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human MET by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-MET recombinant monoclonal antibody (orb1806454). Secondary: HRP-conjugated goat anti-rabbit IgG.
ICC, IF, IHC-P, WB | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse, Rabbit | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating