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Product Overview
Product Name IL6 antibody
Catalog Number orb10911
Species/HostRabbit
ReactivityHuman
ConjugationUnconjugated
Tested applicationsELISA, IF, IHC-P
Immunogen KLH conjugated synthetic peptide derived between 36-40 amino acids of human IL6
Target IL6
Alternative Names
Product Properties
Form/Appearance Liquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol
Concentration - 5 μg (in 100 μl): 0.05 mg/ml
- 20 μg (in 100 μl): 0.2 mg/ml
- 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Affinity purified by Protein A
MW 23 kDa
Uniprot ID P05231 (Human); P08505 (Mouse); P20607 (Rat)
NCBI NP_000591.11; NM_000600.31
Entrez 3569
Product Description

Rabbit polyclonal antibody to IL6

Application Notes
Dilution Range ELISA: 1:500-1000, IHC-P: 1:400-800, IF: 1:100-500
Application Notes In-house testing of several lysates showed problems with WB.
Validation Images
Western Blot on cells and tissues using IL-6 antibody (working concentration 2 ug/ml)
Western Blot on cells and tissues using IL-6 antibody (working concentration 2 ug/ml)
Immunohistochemical analysis of paraffin-embedded human liver carcinoma using IL-6 antibody(5¦Ìg/ml)
Immunohistochemical analysis of paraffin-embedded human liver carcinoma using IL-6 antibody(5¦Ìg/ml)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using IL-6 antibody(5¦Ìg/ml)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using IL-6 antibody(5¦Ìg/ml)
Immunofluorescence analysis of mouse skin tissue using IL6 antibody (dilution of primary antibody - 1:100)
Immunofluorescence analysis of mouse skin tissue using IL6 antibody (dilution of primary antibody - 1:100)
IHC-P staining of mouse skin tissue using IL6 antibody (dilution at 1:100)
IHC-P staining of mouse skin tissue using IL6 antibody (dilution at 1:100)
IHC-P staining of rat skin tissue using anti-IL6 (dilution at 1:100)
IHC-P staining of rat skin tissue using anti-IL6 (dilution at 1:100)
WB analysis of human breast cancer  tissue using anti-IL6 (dilution of primary antibody - 1:5000)
WB analysis of human breast cancer tissue using anti-IL6 (dilution of primary antibody - 1:5000)
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