Histone H2A.X/H2AFX Rabbit Polyclonal Antibody

SKU: orb3119408

Description

The Histone H2A.X/H2AFX/H2A Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, IHC, IP, and WB applications. This antibody targets the Histone H2AX in Human, Mouse, and Rat samples.

Research Area

Cancer Biology, Epigenetics & Chromatin, Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, IHC, IP, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse Immunoprecipitation, 0.5-2 μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human ELISA, 0.1-0.5μg/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Histone H2A.X/H2AFX recombinant protein (Position: S2-T121).
• Target: Histone H2AX
• Molecular Weight: 15 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Carrier-free

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H2A histone family; member X; H2A.X; H2A/X; H2AFX; H2AX; Histone H2A.x

Quick Database Links

Gene Symbol

Histone H2AX

UniProt

P16104