Histone H2A.X (Phospho-Ser139) Rabbit Polyclonal Antibody

SKU: orb14952

Description

This is a rabbit polyclonal antibody targeting Histone H2A.X. It is unconjugated and suitable for use in IF, WB applications. The antibody is reactive with human samples. Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500~1:1000; IF: 1:100~1:200
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide-KLH. Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X
• Target: Histone H2A.X
• Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H2A.X; H2AFX; H2a/x; HIST5-2AX;

Quick Database Links

Gene Symbol

Histone H2A.X

UniProt

P16104