HDAC4 Antibody

• Catalog Number: orb1256072
• Category: Antibodies
• Description: HDAC4 Antibody
• Target: HDAC4
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HDAC4 (NP_006028.2).
• UniProt ID: P56524
• MW: Observed: 140kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: HDAC4, BDMR, AHO3, Histone deacetylase A, HD4, HA6
• Note: For research use only

Western blot analysis of extracts of various cell lines, using HDAC4 antibody (orb1256072) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human breast cancer using HDAC4 Antibody (orb1256072) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast using HDAC4 Antibody (orb1256072) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using HDAC4 Antibody (orb1256072) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using HDAC4 antibody (orb1256072) at dilution of 1:100. Blue: DAPI for nuclear staining.