Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155) Rabbit Polyclonal Antibody

• Catalog Number: orb6132
• Category: Antibodies
• Description: Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155) Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Gallus, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human HDAC4 around the phosphorylation site of Ser246 TA(p-S)EP
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 119 kDa
• Target: HDAC4
• UniProt ID: P56524
• RRID: AB_10938394
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: HDAC4(phospho-Ser246); HDAC5(phospho-Ser259); HDAC
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155)) Polyclonal Antibody, Unconjugated (orb6132) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155)) Polyclonal Antibody, Unconjugated (orb6132) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Sample: Hela (Human) Cell Lysate at 30 ug, 293T (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155) (orb6132) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 119 kD, Observed band size: 119 kD.