HDAC1 Antibody

• Catalog Number: orb1274572
• Category: Antibodies
• Description: HDAC1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 62kDa
• Target: HDAC1
• UniProt ID: Q13547
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: HDAC1 Antibody: HD1, RPD3, GON-10, RPD3L1, Histone
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HDAC1 antibody at 1:1000 dilution.

Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using HDAC1 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using HDAC1 antibody. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug HDAC1 antibody. Western blot was performed from the immunoprecipitate using HDAC1 antibody at a dilition of 1:1000.