HA Tag Antibody

• Catalog Number: orb1530833
• Category: Antibodies
• Description: HA Tag Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ELISA, ICC, IP, WB
• Immunogen: HA Epitope tag (YPYDVPDYA)
• Concentration: 100 µg/ml
• Dilution range: WB - 1:1,000; IP - 20 µl/1 mg lysate; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; ELISA - 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: HA Tag
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of HA-tagged protein by WB from HEK293 transfected with Met-HA-IDO1, HEK293 with IDO1-HA-V5 (Internal Tag), and HEK293 with IDO1-V5-HA (C-terminal Tag) cells, using Rabbit anti-HA Tag recombinant monoclonal antibody at 1:1000.

Detection of HA Tag by western blot of immunoprecipitates from HEK293 transfected with Met-HA-IDO1 lysate.Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody and Rabbit anti-IDO1 recombinant monoclonal antibody .

Detection of HA Tag in FFPE HEK293 cells expressing HA tagged cytoplasmic protein by ICC.Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody.

Detection of HA Tag in FFPE HEK293 cells expressing HA tagged nuclear protein by ICC.Antibody: Rabbit anti-HA Tag recombinant monoclonal antibody.