H4K16ac Antibody

• Catalog Number: orb1257582
• Category: Antibodies
• Description: H4K16ac Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic acetylated peptide around K16 of human Histone H4 (NP_001029249.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200ChIP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 13kDa
• Target: H4K16ac
• UniProt ID: P62805
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200ChIP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K16 antibody (orb1257582) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H4-K16 Rabbit pAb (red, orb1257582) at dilution of 1:100. Blue: DAPI for nuclear staining.