DPF3 Rabbit Polyclonal Antibody (HRP)

• Catalog Number: orb2135286
• Category: Antibodies
• Description: DPF3 is the muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). DPF3 specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Rat
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DPF3
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid
• Purity: Affinity Purified
• Conjugation: HRP
• MW: 40kDa
• UniProt ID: Q92784
• Protein Sequence: Synthetic peptide located within the following region: LYTYPARCWRKKRRLHPPEDPKLRLLEIKPEVELPLKKDGFTSESTTLEA
• NCBI: NP_036206
• Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -2°C to -8°C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Buffer/Preservatives: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose
• Alternative names: CERD4, BAF45C
• Note: For research use only
• Application notes: Predicted homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Expiration Date: 12 months from date of receipt.

WB antibody titration: 0.2-1ug/ml. ELISA titer 1:62500. Positive control: human heart

Rabbit Anti-DPF3 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adult heart. Observed Staining: Nuclear. Primary Antibody Concentration: 1:600. Secondary Antibody: Donkey anti-Rabbit-Cy2/3. Secondary Antibody Concentration: 1:200. Magnification: 20X.