H4 K8Ac; K12Ac; K16Ac Antibody [KM-2], Rabbit IgG

• Catalog Number: orb1671572
• Category: Antibodies
• Description: H4 K8Ac; K12Ac; K16Ac Antibody [KM-2], Rabbit IgG
• Clonality: Recombinant
• Clone Number: KM-2
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: KM-2 antibody was derived from an MRL/lpr lupus mouse.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: histone 4, nucleosome, H4K8Ac, H4K12Ac, H4K16Ac, a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the anti-H4 K8Ac; K12Ac; K16Ac antibody KM-2. HeLa cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r1 version of KM-2 (orb1671572 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed HeLa cells with anti-H4 K8Ac; K12Ac; K16Ac antibody KM-2. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric r version of KM-2 (orb1671572) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue) and the actin stain is phalloidin (red). Panels show from left-right- top-bottom orb1671572- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.