Guinea Pig IgG (H&L) Antibody

• Catalog Number: orb347078
• Category: Antibodies
• Description: Guinea Pig IgG (H&L) antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Guinea pig
• Form/Appearance: Liquid (sterile filtered)
• Concentration: 2.1 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Guinea Pig IgG and Guinea Pig Serum.
• Immunogen: Guinea Pig IgG whole molecule
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:50,000 - 1:125,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application notes: Anti-Guinea Pig IgG antibody has been tested by ELISA and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Alternative names: Rabbit Anti-Guinea Pig IgG, Rabbit Anti Guinea Pig
• Note: For research use only

ELISA Results of Purified Rabbit Anti-Guinea Pig IgG Antibody tested against purified Guinea Pig IgG. Each well was coated in duplicate with 10 µg of Guinea Pig IgG (p/n orb2652762). The working dilution is 1:120000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Rabbit IgG HRP conjugated (p/n orb347654), and TMB substrate (p/n orb348651).