GSK3 beta antibody

• Catalog Number: orb256575
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GSK3 beta
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, IP, WB
• Reactivity: Human, Mouse, Porcine, Rat, Sheep, Zebrafish
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GSK3 beta. The exact sequence is proprietary.
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200, IP: 1:10-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GSK3B
• Entrez: 84027, 2932, 56637
• UniProt ID: P49841, P18266, Q9WV60
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Glycogen synthase kinase-3 beta antibody, ant
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GSK3 beta expression in SGC7901 (A), HEK293T (B), HepG2 (C), PC12 (D) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46 kD)

Immunohistochemical analysis of GSK3 beta staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.