GSK3B Antibody

• Catalog Number: orb1274437
• Category: Antibodies
• Description: GSK3B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GSK3B (NP_001139628.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:1000IF: 1:50 - 1:100IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 46kDa
• Target: GSK3B
• UniProt ID: P49841
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: GSK3B, GSK-3 beta, GSK3 beta, GSK3beta, GSK3beta i
• Note: For research use only
• Application notes: WB: 1:500 - 1:1000IF: 1:50 - 1:100IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using GSK3B antibody (orb1274437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts from normal (control) and GSK3B knockout (KO) HeLa cells, using GSK3B antibody (orb1274437) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunofluorescence analysis of HeLa cells using GSK3B antibody (orb1274437) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of 22Rv1 cells, using 3 ug GSK3B antibody (orb1274437). Western blot was performed from the immunoprecipitate using GSK3B antibody (orb1274437) at a dilution of 1:1000.